Presented above is a widefield fluorescence image revealing the cytokeratin intermediate filament network present in adherent rat kangaroo kidney epithelial cells (PtK2 line). The cells were immunofluorescently labeled with mouse anti-cytokeratin primary antibodies followed by goat anti-mouse secondary antibodies conjugated to the cyanine dye, Cy2. In addition, the specimen was counterstained with MitoTracker Red CMXRos and DAPI, targeting the mitochondrial network and nuclei, respectively. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.