Presented above is a confocal image revealing the intricate network structure of glial fibrillary acidic protein and vimentin intermediate filaments in a thin (8-micrometer) section of rat brain hippocampus (sagittal section). The section was stained as detailed below using a cocktail of mouse anti-vimentin and rabbit anti-GFAP primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively. Cell nuclei were counterstained with DRAQ5. The specimen was imaged with a 60x oil immersion objective (without zoom) using a 488-nanometer argon-ion laser (Alexa Fluor 488), a 543-nanometer helium-neon laser (Alexa Fluor 568), and a 633-nanometer helium-neon laser (DRAQ5; pseudocolored cyan). The brain section image was acquired in grayscale and subsequently pseudocolored with hues approximating the fluorescence emission spectra of the respective probes, with the exception as noted above.