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Applications in Confocal Microscopy
Fluorescence Resonance Energy Transfer (FRET) Literature Sources
Understanding the dynamic interactions between proteins within living cells is fundamental to a basic knowledge of the underlying concepts that guide molecular and cellular biology. Over the past few years, the rapid development of fluorescent proteins and their application as fusion products and biosensors has significantly expanded the molecular toolkit available for probing the mysteries of cellular physiology and pathology. In this regard, fluorescence (or Förster) resonance energy transfer (FRET) is emerging as a powerful optical microscopy technique for examining physiological processes with high temporal and spatial resolution. The references listed below highlight important literature sources for review articles and original research reports on the construction and applications of fluorescent proteins for resonance energy transfer experiments.
Bacskai, B. J., Hochner, B., Mahaut-Smith, M., Adams, S. R., Kaang, B. K., Kandel, E. R., and Tsien, R. Y., Spatially resolved dynamics of cAMP and protein kinase A subunits in Aplysia sensory neurons., Science 260: 222-226 (1993). | PubMed |
Bastiaens, P. I. H. and Jovin, T. M., Fluorescence resonance energy transfer microscopy., in Cell Biology: A Laboratory Handbook, Volume 3, Celis, J. E. (ed.), Academic Press, New York, pages 136-146 (1998).
| Amazon |
Bastiaens, P. I. H., Majoul, I. V., Verveer, P. J., Soeling, H. D., and Jovin, T. M., Imaging the intracellular trafficking and state of the AB5 quaternary structure of cholera toxin., The EMBO Journal 15: 4246-4253 (1996). | PubMed |
Bastiaens, P. I. H. and Pepperkok, R., Observing proteins in their natural habitat: The living cell., Trends in Biochemical Sciences 25: 631-637 (2000). | PubMed |
Berland, K. M., Quantifying molecular interactions with fluorescence correlation spectroscopy., in Molecular Imaging: FRET Microscopy and Spectroscopy, Periasamy, A. and Day, R. N. (eds.), Oxford University Press, New York, pages 272-283 (2005).
| Molecular Imaging |
Berney, C. and Danuser, G., FRET or No FRET: A quantitative comparison., Biophysical Journal 84: 3992-4010 (2003). | PubMed |
Botts, J., Takashi, R., Torgerson, P., Hozumi, T., Muhlrad, A., Mornet, D., and Morales, M. F., On the mechanism of energy transduction in myosin subfragment 1., Proceedings of the National Academy of Sciences, USA 81: 2060-2064 (1984). | PubMed |
Cardullo, R. A., Mungavon, R. M., and Wolf, D. E., Imaging membrane organization and dynamics., in Biophysical and Biochemical Aspects of Fluorescence Spectroscopy, Dewey, T. G. (ed.), Springer, New York, pages 231-260 (1991). | Amazon |
Cardullo, R. A. and Parpura, V., Fluorescence resonance energy transfer microscopy: Theory and Instrumentation., Methods in Cell Biology 72: 415-430 (2003). | PubMed |
Centonze, V. E., Sun, M., Masuda, A., Gerritsen, H., and Herman, B., Fluorescence resonance energy transfer imaging microscopy., Methods in Enzymology 360: 542-560 (2003). | Methods in Enzymology |
Chan, F. K.-M., Siegel, R. M., Zacharias, D., Swofford, R., Holmes, K. L., Tsien, R. Y., and Lenardo, M. J., Fluorescence resonance energy transfer analysis of cell surface receptor interactions and signaling using spectral variants of the green fluorescent protein., Cytometry 44: 361-368 (2001). | PubMed |
Chen, Y., Elangovan, M., and Periasamy, A., FRET data analysis: The algorithm., in Molecular Imaging: FRET Microscopy and Spectroscopy, Periasamy, A. and Day, R. N. (eds.), Oxford University Press, New York, pages 126-145 (2005). | Molecular Imaging |
Chen, Y., Mills, J. D., and Periasamy, A., Protein localization in living cells and tissues using FRET and FLIM., Differentiation 71: 528-541 (2003). | PubMed |
Chen, Y. and Periasamy, A., Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization., Microscopy Research and Technique 63: 72-80 (2004). | PubMed |
Chen, Y. and Periasamy, A., Time-correlated single-photon counting fluorescence lifetime imaging - FRET microscopy for protein localization., in Molecular Imaging: FRET Microscopy and Spectroscopy, Periasamy, A. and Day, R. N. (eds), Oxford University Press, New York, 239-259 (2005). | Molecular Imaging |
Clegg, R. M., fluorescence resonance energy transfer., in Fluorescence Imaging Spectroscopy and Microscopy, Wang, X. F. and Herman, B. (eds.), John Wiley and Sons, Inc., New York, pages 179-252 (1996). | Amazon |
Clegg, R. M., Fluorescence resonance energy transfer and nucleic acids., Methods in Enzymology 211: 353-388 (1992). | Methods in Enzymology |
Day, R. N., Visualization of Pit-1 transcription factor interactions in the living cell nucleus by fluorescence resonance energy transfer microscopy., Molecular Endocrinology 12: 1410-1419 (1998). | PubMed |
Day, R. N., Nordeen, S. K., and Wan, Y., Visualizing protein-protein interactions in the nucleus of the living cell., Molecular Endocrinology 13: 517-526 (1999). | PubMed |
Day, R. N., Periasamy, A., and Schaufele, F., Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus., Methods 25: 4-18 (2001). | PubMed |
Day, R. N. and Piston, D. W., Spying on the hidden lives of proteins., Nature Biotechnology 17: 425-426 (1999). | PubMed |
Derdowski, A., Ding, L., and Spearman, P., A novel fluorescent resonance energy transfer assay demonstrates that the Human Immunodeficiency Virus type 1 Pr55Gag I Domain mediates Gag-Gag interactions., Journal of Virology 78: 1230-1242 (2004).
| PubMed |
Domanov, Y. A. and Gorbenko, G. P., Analysis of resonance energy transfer in model membranes: Role of orientational effects., Biophysical Chemistry 99: 143-154 (2002).
| PubMed |
Dos Remedios, C. G. and Moens, P. D. J., Fluorescence resonance energy transfer spectroscopy is a reliable "ruler" for measuring structural changes in proteins., Journal of Structural Biology 115: 175-185 (1995).
| PubMed |
Elangovan, M., Day, R. N., and Periasamy, A., Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cell., Journal of Microscopy 205: 3-14 (2002). | PubMed |
Elangovan, M., Day, R. N., and Periasamy, A., Dynamic imaging using fluorescence resonance energy transfer., BioTechniques 32: 1260-1265 (2002).
| PubMed |
Elangovan, M., Wallrabe, H., Chen, Y., Day, R. N., Barroso, M., and Periasamy, A., Characterization of one- or two-photon excitation fluorescence resonance energy transfer microscopy., Methods 29: 58-73 (2003). | PubMed |
Erickson, M. G., Moon, D. L., and Yue, D. T., DsRed as a potential FRET partner with CFP and GFP., Biophysical Journal 85: 599-611 (2003). | PubMed |
Fehr, M., Okumoto, S., Deuschle, K., Lager, I., Looger, L. L., Persson, J., Kozhukh, L., Lalonde, S., and Frommer, W. B., Development and use of fluorescent nanosensors for metabolite imaging in living cells., Biochemical Society Transactions 33: 287-290 (2005). | PubMed |
Forde, T. S. and Hanley, Q. S., Following FRET through five energy transfer steps: spectroscopic photobleaching, recovery of spectra, and a sequential mechanism of FRET., Photochemical and Photobiological Sciences 4: 609-616 (2005). | PubMed |
Förster, T., Intermolecular energy migration and fluorescence., Annalen der Physik (Leipzig) 2: 55-75 (1948). | Ann Phys |
Förster, T., Delocalized excitation and excitation transfer., in Modern Quantum Chemistry, Sinanoglu, O. (ed.), Academic Press, New York, pages 93-137 (1965).
| Abe Books |
Gadella, T. W. J. and Jovin, T. M., Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation., The Journal of Cell Biology 129: 1543-1558 (1995). | PubMed |
Galperin, E., Verkhusha, V. V., and Sorkin, A., Three-chromophore FRET microscopy to analyze multiprotein interactions in living cells., Nature Methods 1: 209-217 (2004).
| PubMed |
Gautier, I., Tramier, M., Durieux, C., Coppey, J., Pansu, R. B., Nicolas, J. C., Kemnitz, K., and Coppey-Moisan, M., Homo-FRET microscopy in living cells to measure monomer-dimer transition of GFP-tagged proteins., Biophysical Journal 80: 3000-3008 (2001).
| PubMed |
Gordon, G. W., Berry, G., Liang, X. H., Levine, B., and Herman, B., Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy., Biophysical Journal 74: 2702-2713 (1998). | PubMed |
Gryczynski, Z., Gryczynski, I., and Lakowicz, J. R., Basics of Fluorescence and FRET., in Molecular Imaging: FRET Microscopy and Spectroscopy, Periasamy, A. and Day, R. N. (eds.), Oxford University Press, New York, pages 21-56 (2005). | Molecular Imaging |
Harpur, A. G., Wouters, F. S., and Bastiaens, P. I. H., Imaging FRET between spectrally similar GFP molecules in single cells., Nature Biotechnology 19: 167-169 (2001).
| PubMed |
Haustein, E., Jahnz, M., and Schwille, P., Triple FRET: A tool for studying long-range molecular interactions., ChemPhysChem 4: 745-748 (2003). | PubMed |
He, L., Gramer, A. C., Wu, X., and Lipsky, P. E., TRAF3 forms heterotrimers with TRAF2 and modulates its ability to mediate NF-kappa B activation., The Journal of Biological Chemistry 279: 55855-55865 (2004). | PubMed |
Heim, R., Green fluorescent protein forms for energy transfer., Methods in Enzymology 302: 408-423 (1999). | PubMed |
Herman, B., Resonance energy transfer microscopy., Methods in Cell Biology 30: 219-243 (1989). | PubMed |
Hink, M. A., Visser, N. A., Borst, J. W., van Hoek, A., and Visser, A. J. W. G., Practical use of corrected fluorescence excitation and emission spectra of fluorescent proteins in Förster resonance energy transfer (FRET) studies., Journal of Fluorescence 13: 185-188 (2003). | J Fluorescence |
Hohng, S. and Ha, T., Single-molecule FRET., in Molecular Imaging: FRET Microscopy and Spectroscopy, Periasamy, A. and Day, R. N. (eds.), Oxford University Press, New York, pages 165-179 (2005). | Molecular Imaging |
Hoppe, A., Christensen, K., and Swanson, J. A., Fluorescence resonance energy transfer-based stoichiometry in living cells., Biophysical Journal 83: 3652-3664 (2002).
| PubMed |
Jares-Erijman, E. A. and Jovin, T. M., FRET imaging., Nature Biotechnology 21: 1387-1395 (2003). | PubMed |
Karasawa, A., Araki, T., Nagai, T., Mizuno, H., and Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer., Biochemical Journal (Great Britain) 381: 307-312 (2004).
| PubMed |
Karpova, T. S., Baumann, C. T., He, L., Wu, X., Grammer, A., Lipsky, P., Hager, G. L., and McNally, J. G., Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser., Journal of Microscopy 209: 56-70 (2003). | PubMed |
Keating, E., Brown, C. M., and Petersen, N. O., Mapping molecular interactions and transport in cell membranes by image correlation spectroscopy., in Molecular Imaging: FRET Microscopy and Spectroscopy, Periasamy, A. and Day, R. N. (eds.), Oxford University Press, New York, pages 284-301 (2005). | Molecular Imaging |
Kenworthy, A. K., Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy., Methods 24: 289-296 (2001). | PubMed |
Kenworthy, A. K., Photobleaching FRET Microscopy., in Molecular Imaging: FRET Microscopy and Spectroscopy, Periasamy, A. and Day, R. N. (eds.), Oxford University Press, New York, pages 146-164 (2005). | Molecular Imaging |
Kenworthy, A. K., Petranova, N., and Edidin, M., High-resolution FRET microscopy of cholera toxin B-subunit and GPI-anchored proteins in cell plasma membranes., Molecular Biology of the Cell 11: 1645-1655 (2000). | PubMed |
Kikuchi, K., Small molecule-based FRET sensors which enable ratiometric imaging of living cells., Bioimages 12: 55-60 (2004). | Bioimages |
Kretsinger, R. H., Proteins and the flow of information in cellular function., in Molecular Imaging: FRET Microscopy and Spectroscopy, Periasamy, A. and Day, R. N. (eds.), Oxford University Press, New York, pages 1-20 (2005). | Molecular Imaging |
Laib, S. and Seeger, S., FRET studies of the interaction of dimeric cyanine dyes with DNA., Journal of Fluorescence 14: 187-191 (2004). | PubMed |
Lidke, D. S., Nagy, P., Barisas, B. G., Heintzmann, R., Post, J. N., Lidke, K. A., Clayton, A. H. A., Arndt-Jovin, D. J., and Jovin, T. M., Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET)., Biochemical Society Transactions 31: 1020-1027 (2003). | PubMed |
Mattheyses, A. L., Hoppe, A. D., and Axelrod, D., Polarized fluorescence resonance energy transfer microscopy., Biophysical Journal 87: 2787-2797 (2004). | PubMed |
Majoul, I., Analyzing the action of bacterial toxins in living cells with fluorescence resonance energy transfer (FRET)., International Journal of Medical Microbiology 293: 495-503 (2004). | PubMed |
Mills, J. D., Stone, J. R., Okonkwo, D. O., Periasamy, A., and Helm, G. A., Multiphoton FRET microscopy for protein localization in tissue., in Molecular Imaging: FRET Microscopy and Spectroscopy, Periasamy, A. and Day, R. N. (eds.), Oxford University Press, New York, pages 112-125 (2005). | Molecular Imaging |
Mills, J. D., Stone, J. R., Rubin, D. G., Melon, D. E., Okonkwo, D. O., Periasamy, A., and Helm, G. A., Illuminating protein interactions in tissue using confocal and two-photon excitation fluorescent resonance energy transfer microscopy., Journal of Biomedical Optics 8: 347-356 (2003). | PubMed |
Mitra, R. D., Silva, C. M., and Youvan, D. C., Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein., Gene 173: 13-17 (1996). | PubMed |
Miyawaki, A., Visualization of the spatial and temporal dynamics of intracellular signaling., Developmental Cell 4: 295-305 (2003). | PubMed |
Miyawaki, A., Sawano, A., and Kogure, T., Lighting up cells: labeling proteins with fluorophores., Imaging in Cell Biology 5: S1-S7 (2003). | PubMed |
Miyawaki, A. and Tsien, R. Y., Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein., Methods in Enzymology 327: 472-500 (2000). | Methods in Enzymology |
Mizuno, H., Sawano, A., Eli, P., Hama, H., and Miyawaki, A., Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer., Biochemistry 40: 2502-2510 (2001). | PubMed |
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| Molecular Imaging |
Ng, T., Squire, A., Hansra, G., Bornancin, F., Prevostel, C., Hanby, A., Harris, W., Barnes, D., Schmidt, S., Mellor, H., Bastiaens, P. I. H., and Parker, P. J., Imaging protein kinase C-alpha activation in cells., Science 283: 2085-2089 (1999). | PubMed |
Nguyen, A. W. and Daugherty, P. S., Evolutionary optimization of fluorescent proteins for intracellular FRET., Nature Biotechnology 23: 355-360 (2005). | PubMed |
Patel, R. C., Kumar, U., Lamb, D. C., Eld, J. S., Rocheville, M., Grant, M., Rani, A., Hazlett, T., Patel, S. C., Gratton, E., and Patel, Y. C., Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells., Proceedings of the National Academy of Sciences, USA 99: 3294-3299 (2002). | PubMed |
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| PubMed |
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| PubMed |
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| ChemPhysChem |
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| Molecular Imaging |
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