Human Bone Osteosarcoma Cells (U-2 OS Line)

Histones present in the nuclei of cancerous bone cells (U-2 OS line) were immunofluorescently labeled with primary anti-histone mouse monoclonal antibodies followed by goat anti-mouse Fab heavy and light chain fragments conjugated to Cy3. In addition, the specimen was simultaneously stained for filamentous actin with Alexa Fluor 350 conjugated to phalloidin, and for peroxisomes with Cy2 conjugated to goat secondary antibodies that target rabbit anti-PMP 70 (peroxisomal membrane protein).