Human Bone Osteosarcoma Cells (U-2 OS Line)

The adherent culture of human osteosarcoma (U-2 OS) cells illustrated in this section was stained with Oregon Green 488 conjugated to wheat germ agglutinin, a plant-derived lectin that targets the Golgi apparatus, as well as Alexa Fluor 568 conjugated to phalloidin for cytoskeletal actin. Nuclei were labeled with the ultraviolet-absorbing probe DAPI.