Human Bone Osteosarcoma Cells (U-2 OS Line)

In a double immunofluorescence labeling protocol, a culture of human bone cancer (U-2 OS) cells was treated with a cocktail of mouse anti-histone (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies. The target proteins were subsequently visualized with goat anti-mouse and anti-rabbit secondary antibodies conjugated to Texas Red and Alexa Fluor 488, respectively. The filamentous actin cytoskeletal network was counterstained with Alexa Fluor 350 conjugated to phalloidin, a bicyclic peptide isolated from the death cap mushroom (Amanita phalloides).