Human Bone Osteosarcoma Cells (U-2 OS Line)

Nuclear histone proteins were targeted in a culture of human osteosarcoma cells with mouse anti-histone (pan) monoclonal antibodies, which were imaged with goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy2 (labeling the nucleus). The specimen was simultaneously labeled for peroxisomes with Rhodamine Red conjugated to goat secondary antibodies that target rabbit anti-PMP 70 (peroxisomal membrane protein 70). Alexa Fluor 350 conjugated to phalloidin was utilized to counterstain the cytoskeletal F-actin network.