Male Rat Kangaroo Kidney Epithelial Cells (PtK2)

The technique of double immunofluorescence was employed to simultaneously label an adherent culture of rat kangaroo kidney cells with mouse anti-tubulin and rabbit anti-beta-catenin primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Marina Blue (tubulin) and Rhodamine Red-X (beta-catenin), respectively. The culture was counterstained with SYTOX Green, targeting the DNA present in the cell nucleus.