Male Rat Kangaroo Kidney Epithelial Cells (PtK2)

In a manner similar to the previous culture, the extensive microtubular network present in PtK2 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy3. The epithelial cells were also stained with DAPI, which preferentially binds to nuclear DNA.