Male Rat Kangaroo Kidney Epithelial Cells (PtK2)

A culture of rat kangaroo kidney cells was immunofluorescently labeled with primary anti-beta-catenin rabbit monoclonal antibodies followed by goat anti-rabbit secondary antibody fragments (Fab) conjugated to Rhodamine Red-X. Members of the catenin family of peripheral cytosolic proteins bind selectively to the highly conserved cytoplasmic tail domain of the cell-to-cell adhesion cadherin proteins. In addition, the specimen was subsequently counterstained for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin, and for DNA in the cell nucleus with Hoechst 33258.