Male Rat Kangaroo Kidney Epithelial Cells (PtK2)

In order to label the intermediate filaments in a log phase adherent PtK2 culture, the fixed and permeabilized cells were blocked and treated with mouse anti-vimentin (porcine eye lens) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Texas Red-X. Filamentous actin was visualized with phalloidin conjugated to Alexa Fluor 488, while the nuclei were counterstained with Hoechst 33258.