Horse Dermal Fibroblast Cells (NBL-6)

NBL-6 dermal fibroblast cells were fixed with paraformaldehyde, permeabilized, and treated with a mixture of rabbit (anti-giantin) and mouse (anti-vimentin; pan) primary antibodies, followed by secondary antibodies conjugated to Oregon Green 488 and Texas Red, respectively. Cell nuclei were counterstained with the DNA-selective bisbenzimide dye, Hoechst 33342.