Indian Muntjac Deer Skin Fibroblast Cells

The culture of Muntjac cells illustrated in this section was triple-labeled using double immunofluorescence and a phallotoxin. Nuclei were visualized with mouse anti-histones (core) primary antibodies, while the Golgi complex was stained with rabbit anti-giantin antibodies. Secondary antibodies were goat anti-mouse and anti-rabbit conjugated to Texas Red and Oregon Green 488, respectively to produce red nuclei and green Golgi cisternae. The filamentous actin network was counterstained with Alexa Fluor 350 conjugated to phalloidin.