Indian Muntjac Deer Skin Fibroblast Cells

A culture of Indian Muntjac fibroblast cells was transfected with a DsRed-Mitochondria plasmid subcellular localization vector to target cellular mitochondria. Stable transfectants were isolated and grown into log phase before being fixed, permeabilized, and labeled with DAPI and Alexa Fluor 488 conjugated to phalloidin, targeting DNA in the cell nucleus and the F-actin cytoskeletal network, respectively.