Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

The cytokeratin intermediate filament network in a culture of MDOK cells was visualized by fixing an adherent culture in methanol, followed by treatment with mouse anti-cytokeratin (pan) monoclonal antibodies and goat anti-mouse Fab fragments conjugated to Cy2. Prior to fixation, the cells were exposed to MitoTracker Red CMXRos for one hour, and the culture was subsequently counterstained with Hoechst 33258 to image DNA in the nuclei.