Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

Immunofluorescence with mouse anti-alpha-tubulin was employed to visualize distribution of the microtubule network in dividing Madin-Darby ovine kidney epithelial cell cultures. The secondary antibody (goat anti-mouse IgG) was conjugated to Alexa Fluor 568 and mixed with Alexa Fluor 350 conjugated to phalloidin to simultaneously image tubulin and the actin cytoskeleton. Nuclei were counterstained with SYTOX Green.