Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

An adherent MDOK cell culture in log phase growth was treated with MitoTracker Red CMXRos for one hour before being fixed and permeabilized. The cells were subsequently blocked with bovine serum albumen and incubated with Alexa Fluor 350 conjugated to phalloidin, followed by counterstaining with SYTOX Green.