Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

A fixed and permeabilized adherent culture of MDOK cells was treated with a cocktail of concanavalin A conjugated to Texas Red and Alexa Fluor 488 conjugated to phalloidin to label the endoplasmic reticulum and filamentous actin networks, respectively. The nuclei were subsequently counterstained with Hoechst 33258.