Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

A log phase adherent culture of MDOK cells was fixed and permeabilized with a cocktail of paraformaldehyde, Triton-X100, and glutaraldehyde before being blocked with 10-percent normal goat serum. Subsequent treatment with mouse anti-vimentin monoclonal primary antibodies was followed by goat anti-mouse secondary antibodies (IgG) conjugated to Texas Red-X. In addition, the filamentous actin was labeled with Oregon Green 488 conjugated to phalloidin and the nuclei counterstained with Hoechst 33258.