Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

In a double immunofluorescence experiment similar one described above, a culture of Madin-Darby ovine kidney cells was fixed, permeabilized, and blocked with 10-percent goat serum before being treated with a cocktail of mouse anti-histones (pan) and rabbit anti-giantin primary antibodies. The target organelles were visualized with goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Texas Red and Alexa Fluor 488, respectively. The filamentous actin cytoskeletal network was imaged with Alexa Fluor 350 conjugated to phalloidin.