Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

A comparison between the microtubule and actin networks in MDOK cells was conducted by treating a fixed and permeabilized adherent culture with mouse anti-tubulin primary antibodies, followed by a cocktail of goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568 along with phalloidin conjugated to Alexa Fluor 350 (targeting the microtubule and filamentous actin networks, respectively). Nuclei were counterstained with SYTOX Green.