Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

In order to visualize the interrelationship between the microtubule and mitochondrial networks in MDOK cells, a rapidly growing adherent culture was stained with MitoTracker Red CMXRos, fixed, permeabilized, and then treated with mouse primary antibodies to tubulin. The culture was subsequently labeled with goat anti-mouse Fab antibody fragments conjugated to fluorescein and counterstained with Hoechst 33258 (targeting DNA in the nucleus).