Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)

Nuclear histone proteins were targeted in a culture of Madin-Darby ovine kidney cells with mouse anti-histone (pan) monoclonal antibodies, which were imaged with goat anti-mouse Fab fragments conjugated to Texas Red (labeling the nucleus). The specimen was simultaneously labeled for peroxisomes with Alexa Fluor 488 conjugated to goat secondary antibodies that target rabbit anti-PMP 70 (peroxisomal membrane protein 70). Alexa Fluor 350 conjugated to phalloidin was utilized to counterstain the cytoskeletal F-actin network.