Madin-Darby Canine Kidney Epithelial Cells (MDCK Line)

Simultaneous localization of tight junctions and the nuclear pore complex proteins (NPCP) was performed with a double immunofluorescence experiment with MDCK cells using mouse anti-NPCP and rabbit anti-ZO-3 primary antibodies. The subcellular targets were visualized using goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 488 and Alexa Fluor 568, respectively. DNA in the nuclei was counterstained using Hoechst 33258.