Madin-Darby Canine Kidney Epithelial Cells (MDCK Line)

A fixed and permeabilized adherent culture of Madin-Darby canine kidney cells was immunofluorescently labeled with primary mouse anti-cytokeratin antibodies, followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy2. Before fixation, the culture was treated for one hour with MitoTracker Red CMXRos, and after the antibody treatments, the nuclei were counterstained with Hoechst 33258.