Pig Kidney Epithelial cells (LLC-PK1)

Many epithelial cell cultures are good candidates for immunofluorescence labeling with cytokeratin. The LLC-PK1 culture featured in this section was treated with mouse anti-cytokeratin (pan) primary antibodies followed by goat anti-mouse secondaries conjugated to Texas Red. Simultaneously, the Golgi complex was visualized with rabbit anti-giantin primaries and goat anti-rabbit secondaries conjugated to Oregon Green 488. Nuclei were counterstained with Hoechst 33342.