Pig Kidney Epithelial cells (LLC-PK1)

In order to target the cytoskeletal F-actin network and nuclei present in a LLC-PK1 monolayer culture, the cells were first stained with Alexa Fluor 488 conjugated to phalloidin and propidium iodide, respectively. The culture was also labeled with the probe Alexa Fluor 350 conjugated to wheat germ agglutinin, a lectin that selectively binds to sialic acid residues, which are found in both mucoproteins and glycoproteins.