Tahr Ovary Epithelial Cells (HJ1.Ov Line)

Using a secondary antibody fluorophore combination reversed from that in the section linked directly above (but the same primary cocktail), a second monolayer culture of HJ1.Ov cells was triple-labeled using double immunofluorescence and a phallotoxin. Nuclei were visualized with mouse anti-NPCP (nuclear pore complex protein) primary antibodies, while the Golgi complex was stained with rabbit anti-giantin antibodies. Secondary antibodies were goat anti-mouse and anti-rabbit conjugated to Alexa Fluor 568 and 488, respectively to produce red nuclei and green Golgi cisternae. The filamentous actin network was counterstained with Alexa Fluor 350 conjugated to phalloidin.