Human Cervical Adenocarcinoma Cells (HeLa Line)

In a double immunofluorescence labeling experiment, an adherent culture of HeLa cells was treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively, to target the nuclear histone proteins and peroxisomes. The filamentous actin network was imaged with Alexa Fluor 350 conjugated to phalloidin.