Human Cervical Adenocarcinoma Cells (HeLa Line)

A culture of HeLa carcinoma cells was transfected with a pEGFP-Nucleus plasmid subcellular localization vector. Plasmid pEYFP-Nucleus vector gene product expression in various cell types occurs due to the efficient intracellular translation of a fusion nucleotide sequence combining the enhanced green fluorescent protein domain with three tandem repeats of the nuclear localization signal (NLS) from simian virus 40 (SV40) large T-antigen. In addition, the cells were labeled with Alexa Fluor 568 conjugated to phalloidin in order to target the cytoskeletal filamentous actin network.