Mink Uterus Endometrium Epithelial Cells (GMMe Line)

In what has become a favorite standard fluorescence staining protocol, a log phase monolayer adherent culture of GMMe cells was labeled with MitoTracker Red CMXRos before fixing, and the cells were subsequently permeabilized and stained with Alexa Fluor 488 conjugated to phalloidin, followed by DAPI, a popular DNA-binding counterstain.