Normal African Green Monkey Kidney Fibroblast Cells (CV-1 Line)

A culture of African green monkey kidney cells was immunofluorescently labeled with primary anti-vinculin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy3 (red emission). Vinculin is a protein associated with focal adhesion and adherens junctions, which are membrane-associated complexes that serve as nucleation sites for actin filaments and as crosslinkers between the external medium, plasma membrane, and actin cytoskeleton. The specimen was counterstained for F-actin with Alexa Fluor 488 conjugated to phalloidin, and for DNA with DAPI.