Mongoose Skin Fibroblast Cells (APM)

Using a popular triple-fluorophore staining technique for mitochondria, filamentous actin, and the nucleus, a log phase monolayer culture of APM cells was first treated with MitoTracker Red CMXRos for one hour, and then fixed with medium containing 3.7-percent paraformaldehyde. After permeabilization and blocking with bovine serum albumen, the cells were labeled with Alexa Fluor 488 conjugated to phalloidin and counterstained with Hoechst 33258.