Mongoose Skin Fibroblast Cells (APM)

The microtubule network from the adherent culture of APM fibroblast cells presented in this section was immunofluorescently labeled with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Alexa Fluor 568 (yielding red emission). In addition, the cells were labeled for cytoskeletal F-actin with Alexa Fluor 350 (blue emission) conjugated to phalloidin, and for cell nuclei with SYTOX Green, a classic nucleic acid stain.