Human Lung Carcinoma Cells (A-549 Line)

In order to visualize the relationship between the Golgi complex and the cytokeratin intermediate filament network, a culture of human lung carcinoma (A-549) cells was immunofluorescently labeled primary anti-cytokeratin (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated Texas Red. Golgi bodies were simultaneously targeted with rabbit anti-giantin primary antibodies, followed by goat anti-rabbit secondaries conjugated to Oregon Green 488. Nuclei were counterstained with Hoechst 33342.