Human Lung Carcinoma Cells (A-549 Line)

A-549 cells were immunofluorescently labeled with primary anti-cytokeratin (an intermediate filament protein) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Marina Blue. MitoTracker Red CMXRos (mitochondria) and SYTOX Green (nuclei) were also used to counterstain the carcinoma cell culture.