Human Lung Carcinoma Cells (A-549 Line)

After transiently transfecting a log phase culture of A-549 cells (DsRed2-Mitochondria; 10-percent transfection efficiency), the culture was washed, fixed, permeabilized, and blocked with bovine serum albumen. The cells were subsequently labeled with Alexa Fluor 488 conjugated to phalloidin and counterstained with DAPI. Note that the two central cells in the image are the only individuals expressing the chimeric protein.