Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)

A culture of rat thoracic aorta cells was fixed, permeabilized, and blocked with 10-percent normal goat serum in phosphate-buffered saline prior to immunofluorescent labeling with rabbit primary antibodies to giantin, a protein resident in the Golgi complex of mammalian cells. The culture was subsequently stained with a mixture of goat anti-rabbit secondary antibody fragments (heavy and light chain) conjugated to Cy2. In addition, the cells were labeled with Alexa Fluor 568 conjugated to phalloidin (targeting the filamentous actin network), and for DNA with Hoechst 33342.