Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)

Rat thoracic aorta (A-10) cells were transfected with a chimeric EGFP plasmid vector that expresses a fluorescent fusion protein targeted at cytoplasmic actin. The fusion protein is incorporated into the growing actin filament network to enable visualization of actin-containing subcellular structures in living and fixed cells. The specimen illustrated in this section was also stained for mitochondria with MitoTracker Red CMXRos, and for DNA in the nucleus with the ultraviolet-absorbing probe DAPI.