Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)

Focal adhesions were visualized in a log phase adherent monolayer culture of A-10 cells by immunofluorescent treatment with mouse anti-vinculin primary antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy2. The actin cytoskeletal network was simultaneously imaged with Alexa Fluor 568 conjugated to phalloidin, and nuclei were counterstained with Hoechst 33258.