Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)

Applying a popular triple fluorophore combination, an adherent log phase culture of rat thoracic aorta cells was treated with MitoTracker Red CMXRos in growth medium for one hour, washed and fixed with paraformaldehyde (prepared in growth medium), permeabilized, and blocked with bovine serum albumen. The cells were subsequently labeled with Oregon Green 488 conjugated to phalloidin and counterstained with Hoechst 33258.