Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)

A culture of adherent A-10 rat thoracic aorta cells was stained with Alexa Fluor 488 conjugated to the lectin concanavalin A, which selectively binds to alpha-mannopyranosyl and alpha-glucopyranosyl residues (primarily in the endoplasmic reticulum). Alexa Fluor 568 conjugated to phalloidin and DAPI were also used to label the culture, targeting filamentous actin and nuclear DNA, respectively.