Embryonic Rat Thoracic Aorta Medial Layer Myoblast Cells (A-10 Line)

In order to label the intermediate filaments in a log phase adherent A-10 culture, the fixed and permeabilized cells were blocked and treated with mouse anti-vimentin (porcine eye lens) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Texas Red-X. Filamentous actin was visualized with phalloidin conjugated to Oregon Green 488, while the nuclei were stained with Hoechst 33258.