Embryonic Swiss Mouse Fibroblast Cells (3T3)Presented in this section is an adherent culture of Swiss mouse embryo fibroblasts that was immunofluorescently labeled with Rhodamine Red conjugated to antibodies directed against peroxisomal membrane protein 70 (PMP 70), an abundant and integral membrane component of peroxisomes. Alexa Fluor 488 conjugated to phalloidin and Hoechst 33258 were simultaneously used to counterstain the culture, targeting F-actin and DNA, respectively. |