Embryonic Swiss Mouse Fibroblast Cells (3T3)

The nuclei of embryonic Swiss mouse fibroblasts in culture were targeted with the nucleic acid probe DAPI, which has an excitation maximum at 358 nanometers and an emission maximum at 461 nanometers when bound to DNA in cell cultures and tissue sections. In addition, the cells were also stained with Alexa Fluor 488 conjugated to phalloidin (filamentous actin) and MitoTracker Red CMXRos (mitochondria).