Embryonic Swiss Mouse Fibroblast Cells (3T3)

Log phase 3T3 cells were transfected with a recombinant plasmid vector containing a chimeric fusion gene product of DsRed fluorescent protein and the mitochondrial targeting sequence from subunit VIII of human cytochrome C oxidase. Stable transfectants were fixed, permeabilized, and treated with phalloidin conjugated to Alexa Fluor 488 before being counterstained with DAPI.