Embryonic Swiss Mouse Fibroblast Cells (3T3)

Log phase Swiss mouse embryo cells were immunofluorescently labeled with primary anti-beta-catenin rabbit monoclonal antibodies followed by goat anti-rabbit secondary antibodies conjugated to Rhodamine Red. In addition, nuclear histone proteins were targeted with anti-histone (pan) monoclonal antibodies, which were imaged with goat anti-mouse IgG (H + L) conjugated to Marina Blue (labeling the nucleus). The specimen was subsequently counterstained for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin.