Immunofluorescently Targeting the Microtubule Network in Human Osteosarcoma Cells

Immunofluorescence with mouse anti-alpha-tubulin was employed to visualize the details of the microtubule network in a log phase monolayer culture of human osteosarcoma cells. The secondary antibody (goat anti-mouse IgG) was conjugated to Alexa Fluor 488. In addition, mitochondria in the cells were targeted with MitoTracker Red CMXRos, a derivative of X-rosamine.