DsRed Fluorescent Protein in Transfected Rabbit Kidney Epithelial Cell Cultures

After transiently transfecting an adherent culture of RK13 rabbit kidney cells (DsRed2-Endoplasmic Reticulum; 10-percent transfection efficiency), the culture was washed, fixed, permeabilized, and blocked with bovine serum albumen. The cells were subsequently labeled with Alexa Fluor 488 conjugated to phalloidin and counterstained with Hoechst 33342 (pseudocolored cyan).