Visualizing the Nuclear Histones and Cytoplasmic Peroxisomes in Indian Muntjac Cell Cultures

A log phase culture of Indian Muntjac cells was treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 647 and Alexa Fluor 488, respectively, to target the nuclear histone proteins and peroxisomes. In addition, the F-actin network was labeled with Alexa Fluor 568 conjugated to phalloidin, a phallotoxin isolated from the death cap mushroom.